The STAT3 inhibitor, LLL12, induces cytoskeletal changes in cultured HUVEC cells.

<p>HUVEC cells cultured in 4-well chamber slides were treated with PBS, VEGF (10 ng/mL) alone, VEGF with DMSO or LLL12 (100 nM) for 18 hrs. The cultures were then probed using anti-β-tubulin primary antibodies (green), and F-actin was stained using phalloidin (red). White arrows highlight F-actin localization at the leading edge, while white arrowheads indicate the curling of microtubules at the cell periphery. 200× magnification. Slice depth = 1 µm. Scale bar = 20 µm. Inset 400× magnification.