Inhibition of UCH-L1 activity alters a-syn puncta size and intensity in hippocampal primary neurons from h-a-syn-GFP tg mice.

<p>Cultured h-a-syn-GFP over expressing hippocampal neurons were treated with LDN for 24 hours. At the end of LDN treatments, neurons were fixed, permeabilized, and immunolabeled with anti-MAP2 and anti-a-syn (Syn211) antibody that only recognizes human a-syn. Representative images from control and LDN-treated neurons (A), scale bar = 20 µM. The mean fluorescence intensity (B) and puncta size (C) in LDN-treated neurons were normalized to those of control neurons. Measurements for immunostainings were made on 10 different fields from 3 independent experiments in both control and LDN-treated neurons. Mean values ± SEM are shown. *P<0.01.