Fis, VapC-1 and VapBC-1 bind the <i>vapB-1</i> TIR.

<p>(A) The sequence of the 50TIR indicating the putative Fis binding site (<i>underline</i>) and the inverted repeat regions (<i>arrows</i>). (B) Gel shift products from samples containing 50TIR alone (<i>lane 1</i>) and 10, 30, 150 or 600 molar ratios of Fis to DNA. (C) Products from samples containing 50TIR alone (<i>lane 1</i>), VapC-1 (<i>lane 2</i>), VapC-1 followed by VapB-1 (<i>lane 3</i>), VapB-1 (<i>lane 4</i>), or the reconstituted VapBC-1 complex (<i>lane 5</i>). VapB-1 and VapC-1 are present in a 150∶1 molar ratio to 50TIR. In VapBC-1 samples, VapB-1 and VapC-1 are at a 3∶1 molar ratio, with a VapC-1∶DNA molar ratio of 150∶1. This ratio is reported since VapC-1 is the DNA binding protein and the actual amount of VapBC-1 complexes cannot be determined. The identity of each band is noted at the right of each gel. Each gel represents one of two independent experiments.