VapB-1 targets VapC-1 to the <i>vapB-1</i> translation initiation region.

<p>Gel shift products from a titration of VapB-1 into samples following VapC-1 binding to (A) 50TIR or (B) 50US at a 100∶1 molar ratio of protein to DNA. The gels show the products of the following samples: VapC-1 and DNA without VapB-1 (<i>lane 1</i>), the addition of Vap B-1 at a 50, 100, 200 or 300 molar ratio with the DNA substrate (<i>lanes 2–5</i>), and VapB-1 only at a 300∶1 protein to DNA ratio (<i>lane 6</i>). The identity of each band is noted at the right of each gel. Each gel represents one of two independent experiments.