Fis interacts non-specifically with the <i>vapB-1</i> TIR.

<p>Gel shift products from a titration of Fis, lacking a polyhistidine tag, with (A) 50TIR, (B) GC50TIR or (C) 50US are shown. The gels show the products of the following samples: DNA only (<i>lane 1</i>) and the addition of Fis at 10, 30, 90 or 300 molar ratios with the DNA substrate (<i>lanes 2–5</i>). The identity of each band is noted at the right of each gel. Each gel represents one of two independent experiments.