Augmentation of phagocytosis by CD44 in Tiam-1 −/− mice.

<p>A) Peritoneal macrophages from wild-type (FVB) and Tiam-1 −/− mice were pre-treated with CD44 mAb IM7.8.1 or rat IgG2b control prior to incubation with apoptotic human PMN. After 30 min phagocytosis of apoptotic PMN was determined by microscopy. Results show mean percentage phagocytosis ± SEM for macrophages derived from 4 separate animals. B) Representative microscopy images showing Tiam1 −/− peritoneal macrophages that have been pre-treated with or without CD44 mAb IM7.8.1 prior to incubation with apoptotic human PMN and then stained for myeloperoxidase activity. Scale bar = 20 µm.