Impaired induction of IFI16 expression in cd44^-/- bone marrow-derived macrophages.

Bone marrow-derived macrophages (BMDMs) from wild type and cd44-/- mice were differentiated as described in Materials and Methods. Subsequently, the macrophages were serum-starved in macrophage medium w/o heat-inactivated FBS for 24 h and stimulated with 10 ng/ml IFN-γ for 6 h (A and B) or transfected with the VACV70mer oligo (C and D). RNA was determined as in Fig 4. Experiments were done in triplicates. For statistics see Materials and methods.