Expansion of myeloid cells with PMN-MDSC immunophenotype.

<p>(A) Flow cytometry analysis displayed increase in CD45⁺CD11b⁺ myeloid cells in lung, spleen, liver and heart. (B) Ly6G, Ly6C, Gr1 markers and forward/side scatter parameters were used to define the following myeloid cell subsets: polymorphonuclear myeloid derived cells (PMN-MDSC), monocytic myeloid derived cells (M-MDSC), tumor-associated macrophages (TAM). Analysis was done in 2–3 month-old mice, about one month after <i>i.p.</i> injection of tamoxifen. Data are representative of at least three experiments with similar results (error bars, SEM); at least three TG and control animals were analyzed in each experiment. TG/Endo, ROSA26.vFLIP;Cdh5(PAC).creER^T2; TG/B-cells, ROSA26.vFLIP;CD19.cre mice used as control.