Expression of ephrin B1 is increased during osteoclast differentiation.

<p>[<b>A–B</b>]: Expression of ephrin B1 is increased during osteoclast differentiation. Osteoclast precursors isolated from non-adherent bone marrow of wild-type (WT) mice were treated with 20 ng/ml of M-CSF and 30 ng/ml of RANKL for 0, 2, 4 and 8 days. The differentiated multinuclear cells (MNCs) were monitored by TRAP staining. The parallel cultures were harvested, and total cellular proteins were extracted for Western blot. [<b>C</b>]: Expression of ephrin B1 is more abundant than ephrin B2 in osteoclasts. Mouse splenocytes were treated with M-CSF only or M-CSF plus RANKL for 4 days. Total RNA was extracted for real-time PCR. Values are fold-change over the expression level of ephrin B2 transcript in undifferentiated precursors, and expressed as mean ± SEM (standard error of mean) (n = 3). A star represents statistical significance of expression level of transcript in MNCs as compared to the precursors or between two groups indicated (P<0.01).