Ephrin B1 deletion in cells of myeloid lineage increases bone resorption.

<p>[<b>A–B</b>]: TRAP staining of osteoclasts at trabecular surface of metaphysis of the femur in WT and KO mice at 21 weeks of age. Bone section was counterstained with methyl green. Arrows denote representative TRAP positive osteoclasts. A: 20× magnification, B: 100× magnification. [<b>C</b>]: Percentage change of TRAP labeled surface/trabecular bone surface (Oc. S/BS) in the bone of ephrin B1 KO mice as compared to WT controls at 21 weeks of age. Values are presented as mean ± SEM (n = 8). [<b>D</b>]: Percentage change of serum TRAP activity of ephrin B1 KO mice as compared to WT controls at 21 weeks of age. Values are presented as mean ± SEM (n = 8). [<b>E–F</b>]: Trabecular bone formation is unaffected in mice with deletion of ephrin B1 in myeloid lineage cells. E: Images of H & E staining of distal femurs of WT and KO mice (20×). F: representative images of calcein double labeled trabecular bone of WT and KO mice (200×). [<b>G–H</b>]: Quantitative data of trabecular bone formation measured at distal femur metaphysis of WT and KO mice at 21 weeks of age. G: Bone formation rate/bone surface (BFR/BS). Values are presented as mean ± SEM (n = 8). H: Mineral apposition rate (MAR). Values are presented as mean ± SEM (n = 8).