Overexpression of ephrin B1 increases osteoblast differentiation and osterix expression.

<p><b>[A]</b> Multiple ephrin B ligands and ephrin receptors are expressed in bone marrow stromal (BMS) cells derived from WT and Tg mice, measured by real-time RT-PCR. <b>[B]</b> EphB2 expression in BMS cells, detected by Western blot analysis. <b>[C]</b> Images and quantitative data of mineralized nodules. <b>[D]</b> Quantitative data for mineralized nodule formation. BMS cells were differentiated for 24 days, followed by alizarin red staining and computerized quantification. Data are means ± SEM for N = 3 (3 mice/genotype). <b>[E]</b> Expression of osterix and collagen 1A1 (Col1) are increased in differentiated BMS cells, detected by Western blot analysis. BMS derived from WT and Tg mice were differentiated in the presence of ascorbic acid and beta-glycophosphate for 6 days prior to harvesting for Western blot analysis. [<b>F & G</b>] Quantitative data of osterix and col1 expression, measured by Western blot analysis. Data are means ± SEM for N = 4 (4 mice/genotype). A star represents statistical significance (P < 0.01) when compared to WT littermate controls.