Growth retardation and peripheral blood morphology in postnatal <i>CALM</i>-deficient mice.

<p>(A) Wild-type (+/+) (left) and <i>CALM</i>-deficient (−/−) (right) mice at 3 weeks of age. (B) Average weight of wild-type (+/+) and <i>CALM</i>-deficient (−/−) mice at postnatal day 28. Data shown represent <i>CALM</i>-deficient males (n = 3), <i>CALM</i>-deficient females (n = 4), wild-type males (n = 36), and wild-type females (n = 35). **, <i>P</i><0.01 vs control. Liver (C), spleen (D) and tibia (E) from wild-type (+/+) or <i>CALM</i>-deficient (−/−) mice at 3 weeks of age. (F) Bone marrow cells were collected by centrifugation. Note that the pellet from <i>CALM</i>-deficient (−/−) mice is small and pale in color. (G) Quantification of TER119-positive bone marrow cells (data represent means±SD; n = 5). *, <i>P</i><0.05 vs. control. (H) Peripheral blood smears from wild-type (+/+) littermates (left panels, a and c) or <i>CALM</i>-deficient (−/−) mice (right panels, b and d) at 3 weeks of age. Magnification: upper panels, 400×; lower panels, 1000×. Scale bar, 50 µm (upper panel) and 20 µm (lower panel).