The hematologic changes of peripheral blood and BM in <i>Npm1^wt/c+</i> mice without signs of myeloproliferation.

<p>Monthly blood collection from wild-type and <i>Npm1^wt/c+</i> mice without signs of myeloproliferation showed increase in the compartment of monocytes (<b>A</b>) but not granulocytes (<b>B</b>), lymphocytes, or platelet (data not shown). Each data point was the average of at least 10 mice. (<b>C</b>) There were significant more monocytes (F4/80⁺/Gr-1⁻) in non-myeloproliferative <i>Npm1^wt/c+</i> mice compared to wild-type mice at age of 18 months (n = 7 each). (<b>D</b>) At age of 12 months (n = 7 each), quantification of marrow cells of HSPC (c-kit⁺/sca-1⁺), MPP (F4/80⁻/c-kit⁺), CMP (F4/80⁺/c-kit⁺), GMP (F4/80⁺/Gr-1⁺), monocytes (F4/80⁺/Gr-1⁻), and granulocytes (F4/80⁻/Gr-1⁺) by flow cytometry revealed significant increase in marrow stem cell and progenitor cell compartments of HSPC, CMP, and GMP, but not MPP. Data are expressed as mean ± SE. * means <i>P</i><0.05; ** <i>P</i>≤0.01.