Analysis of haematopoietic progenitors in the bone marrow and peripheral blood.

<p>(A) There was no significant difference in total bone marrow cell numbers between PECAM-1^−/− and WT mice (n = 21). (B) The percentage of KSL cells in the bone marrow cellular pool was measured by flow cytometry, and no significant difference was observed (n = 22 pairs). Bars in (A) and (B) represent mean values, and in both cases no significant difference was observed. (C) The number of colony forming cells (CFCs) in peripheral blood of mice was determined using methylcellulose assays. Data shown is the mean±S.D. of 6 pairs of littermate mice, **p = 0.008. To measure the ability of PECAM-1^−/− HSCs to mobilise from the bone marrow, mice were treated with a daily injection of G-CSF. No significant difference in the number of CFCs released into the peripheral blood was found. (n = 6 pairs of littermates).