<p><b>A:</b> Representative insulin receptor binding curves for HI(black), B25C-NEM1 (dark gray) B25C-NEM2(gray)and the B25C dimer(light gray). <b>B:</b> Representative metabolic dose response curves for HI(black) and the B25C-dimer (dark gray). Each point on the graph represents the mean ± SD, n = 4 within one assay.</p
Insulin analogues comprising acidic amino acid substitutions at position B10 have previously been sh...
<p>Affinities were determined by insulin competition binding in a scintillation proximity assay; dat...
<p>"10 nM S961" and "100 nM S961" curves: Cells were pretreated for 2h with 10 nM or 100 nM S961, an...
<p>Results are presented as absorbance (arbitrary value) mean ± SE, n = 4. IR, insulin receptor; IGF...
<p>All assays were performed in at least three independent experiments. Data are means ± SD and pres...
<p>Receptor binding affinities for the solubilised IR-A (light teal), IR-B (dark teal) and IGF-IR (g...
<p>Dissociation was measured at different time points and the residual binding expressed as a percen...
<p><b>A:</b> Comparison of full-length MGF (red points and line) and insulin (green points and line)...
<p>Time course of (<b>A</b>) plasma insulin concentration (pM), and (<b>B</b>) plasma glucose (mM) i...
<p>Human insulin (•) or insulin analogue (B10D (♦), B10E (▪), or B10A (▾)) stimulated incorporation ...
The metabolic activity of insulin has been studied extensively in vitro and in vivo, based on the in...
<p>Left Panel: Western blot results for phosphorylated insulin receptor (Tyr 1150/1151) in β2-adrene...
Data from the metabolic assays (and signaling assays; see below) are calculated as stimulation facto...
<p>(<b>A</b>) Western blots of proteins participating in liver insulin signaling. (<b>B</b>) Quantit...
<p>Data are mean ± SD (n = 20). T-test C57Bl6 vs. alb-SREBP-1c: **p<0.01.</p><p>Surrogate indices we...
Insulin analogues comprising acidic amino acid substitutions at position B10 have previously been sh...
<p>Affinities were determined by insulin competition binding in a scintillation proximity assay; dat...
<p>"10 nM S961" and "100 nM S961" curves: Cells were pretreated for 2h with 10 nM or 100 nM S961, an...
<p>Results are presented as absorbance (arbitrary value) mean ± SE, n = 4. IR, insulin receptor; IGF...
<p>All assays were performed in at least three independent experiments. Data are means ± SD and pres...
<p>Receptor binding affinities for the solubilised IR-A (light teal), IR-B (dark teal) and IGF-IR (g...
<p>Dissociation was measured at different time points and the residual binding expressed as a percen...
<p><b>A:</b> Comparison of full-length MGF (red points and line) and insulin (green points and line)...
<p>Time course of (<b>A</b>) plasma insulin concentration (pM), and (<b>B</b>) plasma glucose (mM) i...
<p>Human insulin (•) or insulin analogue (B10D (♦), B10E (▪), or B10A (▾)) stimulated incorporation ...
The metabolic activity of insulin has been studied extensively in vitro and in vivo, based on the in...
<p>Left Panel: Western blot results for phosphorylated insulin receptor (Tyr 1150/1151) in β2-adrene...
Data from the metabolic assays (and signaling assays; see below) are calculated as stimulation facto...
<p>(<b>A</b>) Western blots of proteins participating in liver insulin signaling. (<b>B</b>) Quantit...
<p>Data are mean ± SD (n = 20). T-test C57Bl6 vs. alb-SREBP-1c: **p<0.01.</p><p>Surrogate indices we...
Insulin analogues comprising acidic amino acid substitutions at position B10 have previously been sh...
<p>Affinities were determined by insulin competition binding in a scintillation proximity assay; dat...
<p>"10 nM S961" and "100 nM S961" curves: Cells were pretreated for 2h with 10 nM or 100 nM S961, an...