Altering the expression of miR-146a in microglia.

<p><b>A.</b> MiR-146a over-expression as measured by TaqMan® qRT-PCR in microglia cells after 8, 24, and 48 hrs of treatment with 30 nM (previously optimized, data not shown) of transfected pre-miR-146a in comparison to 30 nM of transfected scrambled negative control miRNA. An average fold change and standard deviation were measured from triplicate experiments. <b>B.</b> MiR-146a knock-down as measured by TaqMan® qRT-PCR in microglia cells after 8, 24, and 48 hrs of treatment with 50 nM (previously optimized, data not shown) of transfected anti-miR-146a in comparison to 50 nM of transfected scrambled negative control miRNA. An average fold change and standard deviation were measured from triplicate experiments.