Overexpression of miR-146b suppresses expression of Smad4, Hmga2 and Notch1 in myoblasts.

<p>(A) C2C12 myoblasts were transfected with 50 nM miRIDIAN miR-146b mimic or cel-miR-67 mimic as control. After 24 hours, cells were lysed for RNA isolation, followed by qRT-PCR to measure mRNA levels for the genes shown. Relative mRNA levels are shown with that of cel-miR-67 as 1. Data shown are the mean ± SD from three independent experiments. One-sample <i>t</i> test was performed. **<i>P</i><0.01. (B) Cells as described in A were lysed after 48 hours of transfection and subjected to Western blotting analysis. Representative results of three independent experiments are shown.