miR-146a attenuates NF-κB activation and reduces inflammatory responses in macrophages.

<p>RAW264.7 cells were transfected with miR-146a mimic and negative control miRNA for 24 h and followed hypoxia/reoxygenation. (A and B) Supernatants were collected to measure TNF-α and IL-6 by ELISA. (C) Cytosolic and nuclear p65 protein were separated using hypotonic buffer. β-actin and lamB1 indicate cytosolic and nuclear fraction, respectively. (D and E) EMSA and luciferase report assay of nuclear P65 activity. Co-culture of RAW264.7 cells/Kupffer cells with hepatocytes and followed H/R. Results are mean ± SD. <b>*</b>, P<0.05 and <b>**</b>,P<0.01,<b>***</b>,P<0.001 versus negative control. H/R, hypoxia/reoxygenation; mNC, negative control miRNA of miR-146a mimic; iNC, negative control miRNA of miR-146a inhibitor.