Distribution of the injected A15 probe 24 h after cerebral ischemic induction.

<p>Areas with high fluorescence intensities were observed over the ischemic region in MCAO mice that received A15 (A), corresponding to the pallor in TTC staining (B). High magnification of the boxed region in A was shown in C (C, 40×). Intense and diffuse Cy5.5 fluorescence from A15 was observed in the ischemic area of the cortex and nearby micro-vessels (↑), whereas fluorescence was scarcely visible in the nonischemic areas. The border between the ischemic and nonischemic area was clearly delineated after TTC (B) and hematoxylin and eosin staining (D).