Detection of GM1, GD1a and GM3 by immunofluorescence staining within the mouse brain following MCAO.

<p>Mice were perfusion fixed with 4% formaldehyde. Coronal brain sections at 10 µm thickness were cut and immunostained with either mouse anti-GM1, GD1a or GM3 (red color). Sections were counterstained with 2 µg/ml Hoechst 33258 (blue color) and imaged with an Olympus Fluoview FV1000 confocal laser scanning microscope. Photomicrographs depict the border area between ischemic infarct core and the peri-infarct area clearly separated by the white colored line. At 7 d reperfusion, the border of the stroke-induced infarct is visible (lower left half). Expression levels of GM1, GD1a and GM3 increased within the infarcted ipsilateral cortex. GM1 and GM3 increases were restricted to the stroke-induced infarct core (abbreviations: ctx, cerebral cortex; cc – corpus callosum; isc, ischemic area).