A mutation in T. brucei 5S rRNA loop C does not affect binding to P34 or P37.

<p>Panel <b>A</b>: A filter binding assay with labeled RNA was performed and binding is expressed in arbitrary units is plotted against protein concentration of P34. Blue diamonds: wild type <i>T. brucei</i> 5S rRNA; red squares: loop C mutant. Panel <b>B</b>: Competition EMSAs were performed using P34, labeled 5S rRNA and unlabeled competitors: wild type β arm, ΔG mutation (indicated with a red arrow in the structure on the left) and ΔU mutation (blue arrow). An asterisk indicates the shift.