<i>T. brucei</i> L5 binds a low percentage of the total nuclear 5S rRNA.

<p>Northern blot analysis of 5S rRNA within immunoprecipitate samples utilizing either anti-L5 or anti-P34/P37 antibodies to immuno-precipitate these proteins and associated 5S rRNA from wild type nuclear extracts. Lane 1: total RNA, lane 2: pre-immune sera control, lane 3: experimental lane, lane 4: supernatant fraction. Lanes 5–8: same as lanes 1–4, respectively, but UV cross-linking was performed prior to immunoprecipitation.