Effects of ethanol and androgenization on oxidative stress.

<p>A. Representative images of 4-HNE stained formalin-fixed paraffin-embedded liver tissue from rats maintained on control Lieber-DeCarli (C-LDC, top panels) or ethanol-LDC (E-LDC, bottom panels) diet. Panels A and D, control females (Ctrl); B and E, androgenized females (Andro); panels C and F, Andro + estrogen (Andro + E2). PT; portal triad. Caramel brown staining indicating 4-HNE positive cells. B. Hepatic lipid peroxidation was assessed by Thiobarbituric Acid Reactive Substances (TBARS) assay. Liver tissue was homogenized and malondialdehyde (MDA) formation measured. TBARS were normalized to total protein. Data are presented as mean values ± SEM. *P<0.05.