Histological examination of liver specimens in rats with alcohol-induced liver injury.

<p>Five animals per group and three H&E sections per animal were analysed. Images and data from the CARV 1 mg/ml, 3 mg/ml, and 5 mg/ml groups are shown in panels A–C, D–F, and G–I, respectively (arrow, fatty changes within hepatocytes;triangle, necrosis area). Negative control group slides are shown in A, D, and G. Alcohol group slides are shown in panels B, E, and H (middle images). In the alcohol-only group, rat livers exhibited steatosis with various degrees of diffuse hepatic steatosis and intralobular, deranged hepatic cord, and necrotic areas. Liver injury (<i>e</i>.<i>g</i>., steatosis and inflammation) persisted in the alcohol-CARV 1 mg/kg group (C). (F, I) Reduced steatosis and hepatocyte regeneration associated with reduced necrosis were observed in the livers of animals in the alcohol-CARV 3 mg/kg and alcohol-CARV 5 mg/kg groups. The neutrophils infiltrate the parenchyma, as seen here, and often surround ballooned hepatocytes (blue arrow: neutrophils and circle: lymphocytes and neutrophils infiltrate, Fig 3H1). Reduced neutrophilic infiltration was observed in the livers of animals in the alcohol-CARV 5 mg/kg groups (Fig 3I1). Magnification 400×, scale bar = 100 μm. (J) Representative samples from each CARV treatment group are shown with graphs summarizing the mean histopathological score of each group. (***<i>p</i> < 0.001 <i>vs</i>. negative control, #<i>p</i> > 0.05, *<i>p</i> < 0.05, and ***<i>p</i> < 0.001 <i>vs</i>. alcohol-only group).