Effects of ethanol and androgenization on CYP450 expression.

<p>A. Quantitative Real-Time PCR analysis of CYP2E1 mRNA expression in control (Ctrl), androgenized (Andro) and Andro + estrogen (Andro + E2) rats maintained on control Lieber-DeCarli (C-LDC) or ethanol-LDC (E-LDC) diet. B. Representative CYP2E1 protein expression was determined by Western blot analysis (upper panel) and quantified by optical integrated volume (lower panel). Data are presented as mean values ± SEM. Standard square root transformation was performed prior to statistical analysis. *P<0.05. C. Quantitative Real-Time PCR analysis of CYP1A2 mRNA expression compared between groups described in A. Data are presented as mean values ± SEM. *P<0.05. D. Representative CYP1A2 protein expression was determined by Western blot analysis (upper panel) and quantified by optical integrated volume (lower panel). RNA and protein expression were normalized to GAPDH.