Whole-cell current recordings of BK in HEK 293 cells.

<p>Depletion of cholesterol by MßCD treatment increased BK current dramatically. (A) Control records of BK current in HEK 293 cells stepped from a holding potential of −70 mV to −40 mV∼+80 mV. (B) The same voltage protocol applied to the same cell after cholesterol depletion by MßCD. (C) shows compiled current-voltage plots before and after MßCD treatment. (D) Data showing control data of stable BK current without MßCD treatment and sequential values of BK current over time, before and after MßCD treatment at the holding potential of +60 mV.