Effects of LDL on BK channel current in BK-HEK 293 cells.

<p><b>A</b>. Voltage-dependent BK current recorded with voltage protocol as shown in the inset in cells treated with LDL (75 µg/mL) or LDL plus MβCD treatment. <b>B</b>. <i>I-V</i> relationships of BK current in control, LDL or LDL plus MβCD treatment (n = 25, *<i>P</i><0.05; **<i>P</i><0.01 vs. control). <b>C</b>. Percentage values compared to control of BK current at +60 mV in control (n = 25), with LDL (n = 32, **<i>P</i><0.01 vs. control), and LDL plus MβCD treatment (n = 10, ^##<i>P</i><0.01 vs. LDL alone). <b>D</b>. Activation variables calculated with <i>I-V</i> relationships of the channel current in panel <b>B</b> were fitted to a Boltzmann function. <b>E</b>. Western immunoblots showing KCa1.1 protein and the auxiliary β1-subunit protein in cells treated with LDL or LDL plus MβCD. <b>F</b>. Mean percentage values of KCa1.1 protein and the auxiliary β1-subunit protein in cells with LDL or LDL plus MβCD (n = 6-9, **<i>P</i><0.01 vs. control, ^#<i>P</i><0.05 vs. LDL alone).