Normal contribution of <i>Hnrpll</i>^thu/thu NKT cells, but a cell autonomous decrease in NK1.1 in the mixed bone marrow chimeras.

<p>Irradiated C57BL/6 mice were reconstituted with an equal mixture of CD45.1-marked wild-type bone marrow and CD45.2-marked <i>Hnrpll</i>^thu/thu bone marrow cells. After hemopoietic reconstitution, recipient mice were analysed for B, NKT and conventional T cell populations. (A) Graph shows the ratio of CD45.2⁺ wild type and <i>Hnrpll</i>^thu/thu derived T cells to CD45.2⁺ wild type and <i>Hnrpll</i>^thu/thu derived B cells in the spleen of chimeric recipient mice. (B) Graph shows the ratio of CD45.2⁺ wild type and <i>Hnrpll</i>^thu/thu derived NKT cells to CD45.2⁺ wild type and <i>Hnrpll</i>^thu/thu derived B cells in the spleen of chimeric recipient mice. (C, D) Representative overlay histograms comparing the expression of NK1.1 on wild type (shaded grey) and <i>Hnrpll</i>^thu/thu (black line) derived NKT cells in the (b) thymus and (c) spleen of individual recipient mice from mixed bone marrow chimeras. Graphs show the geometric mean florescence intensity (MFI) of NK1.1 in the CD45.2⁺ wild type or <i>Hnrpll</i>^thu/thu derived NKT cells in the (b) thymus and (c) spleen of individual recipient mice from mixed bone marrow chimeras. (n = 6 for wild type and n = 5 for <i>Hnrpll</i>^thu/thu). Bars represent the mean ± s.d. values.