Quantitative and phenotypic variation of iVα14 NKT cells in the thymus, spleen and liver of WT, B7-1/2 and CD28 deficient mice.

<p>Total lymphocytes from the thymus (left), spleen (middle) and liver (right) of 8-week old WT (top), B7-1/2 (center) and CD28 (bottom) deficient mice were analyzed. A. The quantification of iVα14 NKT cells. The numbers represent the percentage (Mean±SD, n = 9) of iVα14 NKT cells. B. The expression of CD44 and NK1.1 among the gated iVα14 NKT cells. The numbers represent the percentage (Mean±SD) of NK1.1⁻ iVα14 NKT cells. C. The expression of CD4 and NK1.1 from gated iVα14 NKT cells. The numbers represent the percentage (Mean±SD) of NK1.1⁻CD4⁺ (upper left) and NK1.1⁺CD4⁻ (lower right) iVα14 NKT cells. One representative profile from each group is shown here. The numbers shown on the side of figures are <i>p</i> value between two indicated groups.