Intrinsic requirement of PKC-θ for NKT cell development.

<p>Bone marrow from WT and <i>PKC-θ^−/−</i> donors was adoptively transferred to irradiated WT or <i>PKC-θ^−/−</i> recipients (6 mice per group). Ten weeks after adoptive transfer, NKT cells in thymi were analyzed by flow cytometry. A) CD1d and CD3 NKT cells in thymi of adoptively transferred mice. B) Stage 0 and stage 1–3 NKT cells in thymi of adoptively transferred mice. C) Frequencies of total NKT (left panel), and stage 0 and stage 1–3 NKT cells (right panel) as averaged from 6 recipients of each type of adoptive transfer. (**, P<0.01).