Chlorophyll biosynthetic pathway intermediate contents and ¹O₂ production in ALA-treated WT and <i>PORCx</i> plants.

<p>WT and T-13 plants grown for 28–32 days at 22°C±2°C under 14 h L/10 h D photoperiod (100 µmoles photons m⁻² s⁻¹) were sprayed with ALA (3 mM), dark incubaed for 14 h and exposed to light (100 µmoles photons m⁻² s⁻¹) for 10 min. Leaves were harvested both from dark incubated and light exposed plants, homogenized and their tetrapyrrole contents were monitored by spectrofluorometrically. (<b>A</b>) Pchlide, Proto IX and MP(E) contents of ALA-treated (3 mM) and 14 h-dark-incubated WT and T-13 plants. (<b>B</b>) After dark incubation both WT and T-13 plants were exposed to light (10 min) and their Pchlide, Proto IX and MP(E) were determined. (<b>C</b>) ¹O₂ contents in ALA-treated (+ALA) and untreated (-ALA) WT and T-13 plants. The experiments were repeated 5 times and each data point is the average of 5 replicates. The error bar represents ± SD.