Pigment contents in wild-type and mutant leaves.

(A) Schematic view of the Chl biosynthetic pathway. Urogen III (in red) at the beginning of the pathway shows a significant increase in yellow leaves. CHLG/P illustrates the gene encoding protein catalyzing the reaction of the precursors. A green box represents each component that is significantly decreased or down-regulated in the yellow leaves. Coprogen III had no significant difference between green and yellow leaves. (B) Comparison of the relative content of Chl precursors and photosynthetic pigments between wild-type and mutant. (C) Comparison of the flavonoid contents between wild-type and mutant leaves. Asterisks indicate: (*) P ⩽ 0.05, (**) P ⩽ 0.01. Abbreviations are: Urogen III = uroporphyrinogen III; Coprogen III = coproporphyrinogen III; Proto IX = protoporphyrin IX; Mg-Proto IX = Mg-protoporphyrin IX; Pchlide = protochlorophyllide; Chlide a = chlorophyllide a; Chl a = chlorophyll a; Chl b = chlorophyll b; and Caro = carotenoid.