Content of chlorophyll/heme precursors and their changes during the Rc-induced greening process.

<p>Rate of 5-ALA biosynthesis (A) and content of the chlorophyll precursors, protoporphyrin IX (B), Mg-protoporphyrin IX (C) and Mg-protoporphyrin IX monomethylester (D), in the WT and <i>phyB</i> seedlings and the changes during the Rc-induced greening experiment. Values of 5-ALA biosynthesis rates (A) were estimated from the accumulation of 5-ALA during incubation with levulinic acid for 90 min. Each value represents the mean with standard errors of at least six replicates. Symbols, ** and * indicate statistically significant differences compared to WT at a levels of <i>P</i> < 0.01 and <i>P</i> < 0.05, respectively, calculated by Welch’s <i>t</i>-test. nd, not detected.