Acute βAR stimulation doesn't affect the activity of PI3K/Akt or ERK1/2 in β1/β2DKO mouse heart.

<p>Adult male β₁/β₂ double knockout mice were treated with vehicle control or isoproterenol (ISO, 1.25 mg/kg, i.p.) for 30 min. (A) Left ventricular lysates were subjected to <i>in vitro</i> lipid kinase assay as described above. The bar graph shows the densitometric scanning results of the measurement of cardiac PI3K activities in the control (n = 3) and ISO-treated (n = 3) mice. (B, C) Representative Western blot analyses were performed on LV tissue lysates as described above. The bar graphs show the densitometric scanning results from three seperate experiments. Data are normalized with individual total protein levels.