Signalling events downstream of Gα_i stimulation.

<p>Representative western blot of cardiac tissues of animals treated either with saline or CCh (0.5 mg/kg body weight) for 15 min. (A) Western Blot and densitometry analyses of ventricular homogenates show that Akt phosphorylation was significantly and to a similar extent increased after CCh stimulation in all genotypes (n = 3). Total amount of Akt protein was unaltered and was used as loading control. (B) Western blot showing ERK1/2 phosphorylation and total ERK1/2 expression and bar graphs of combined results expressed as increase in pERK1/2 normalized to total ERK1/2 and compared to saline treated WT mice. The increase of ERK1/2 activation in CCh treated cells was markedly inhibited by Gα_i2 gene deletion compared to WT and Gα_i3^−/− mice (n = 3). *p<0.05 vs. WT NaCl.