Cardiac Akt phosphorylation in increased in myriocin treated DIO mice.

<p>Isolated working mouse hearts were perfused aerobically in the presence of insulin (100 µU/mL ) for determination of, <i>A:</i> Cardiac Akt serine 473 phosphorylation, <i>B:</i> GSK3β serine 9 phosphorylation, and <i>C:</i> AMPK threonine 172 phosphorylation in low fat fed and DIO mice treated with control or myriocin <i>D:</i> Cardiac Akt serine 473 phosphorylation, GSK3β serine 9 phosphorylation, AMPK threonine 172 phosphorylation, and PDK4 expression in control and myriocin treated <i>db/db</i> mouse hearts. Values represent mean ± SE (n = 4–7). Differences were determined using an unpaired Student’s two-tailed t-test or a one-way ANOVA followed by Bonferroni post-hoc analysis. *<i>P</i><0.05, significantly different from DIO control treated mice. ^†<i>P</i> = 0.06.