BITC inhibits migration and invasion of pancreatic cancer cells.

<p><b>A</b>. BITC inhibits migration of BxPC-3 cells. BxPC-3 cells were plated, scratched with pipette tip, and incubated in the absence or presence of 5 µM BITC. Photomicrographs were made at regular intervals using inverted microscope. <b>B</b>. Quantitative representation of migration assay. Wound area in BITC-treated and control cells were quantified by Image J software and presented as mean ± SD of triplicates. p<0.01, statistically significant when compared to corresponding time points in controls cells. <b>C–D</b>. BITC inhibits the invasion of BxPC-3 and PanC-1 cells. Invasion assay was performed using Boyden's chamber. Results are presented as mean ± SD of triplicates. p<0.05, statistically significant when compared controls.