<p>Cryopreserved PBMC were thawed and analysed. Representative FACS plots indicating our gating strategy are shown in (A): following gating on the lymphocyte population as determined by the FSC/SSC profile, we analysed lymphocyte populations. Shown are a representative CD4+ and CD8+ profile and a comparison of Vα24+Vβ11+ invariant NKT cell levels in a progressor (left) and non-progressor. All subjects were analysed for percentages of total T cells (B), CD4+ (C), CD8+ (D), CD4∶CD8 Ratio (E), B cells (F) and NK cells (G). Bars indicate median of 14 progressors and 31 non-progressors at recruitment (0), 9 progressors and 35 non-progressors at 3 months (3) and 9 progressors and 22 non-progressors at 18 months (18). Data were analysed using a Kr...
<p>T cells were separately analyzed for CD4<sup>+</sup> and CD8<sup>+</sup> subpopulations. NKT cell...
Flow cytometry was used to assess mature B lymphocyte populations for each group. Each graph shows n...
<p>(A and B) Absolute numbers of B cells, CD8 T cells, NK cells and CD4 T cells were re-evaluated af...
<p>Data is presented from 30 subjects (17 in the SSG arm and 13 in TM arm represented in circles and...
<p>a) Viable cells were gated based on negative expression of the fixable viability stain (FVS). Tru...
<p>Healthy human donor PBMCs were mock or VZV infected with or without IL-2 (200 U/ml) for 2 days th...
<p>PBMC were obtained from blood samples and stained for CD45, NK and CD3 with fluorescent antibodie...
<p>PBMC were analyzed post cryo preservation by flow cytometry, to determine the <i>ex vivo</i> freq...
<p>FSc-SSc profile was used to distinguish lymphocytes, monocytes and granulocytes (A). Within the l...
A primary goal of the Denton Immunobiology Lab is improving the killing efficacy of natural killer (...
<p>(A) Gating strategy to distinguish NK cells and subgroups CD56<sup>dim</sup> and CD56<sup>bright<...
<p>A) Percentage values of different cell populations (as indicated, including cytokine-induced kill...
<p>Panels A-D: Lymphocyte subsets among patients included in the randomized KTS-1-2008 study, in the...
<p>(A) Shown are isotype control dot plots and CD14 and CD16 expression in freshly obtained and cryo...
The % of CD3 (CD3+CD19-), the % of CD4 (CD3+CD4+), the % of CD8 (CD3+CD8+) and the % of CD19 (CD3-CD...
<p>T cells were separately analyzed for CD4<sup>+</sup> and CD8<sup>+</sup> subpopulations. NKT cell...
Flow cytometry was used to assess mature B lymphocyte populations for each group. Each graph shows n...
<p>(A and B) Absolute numbers of B cells, CD8 T cells, NK cells and CD4 T cells were re-evaluated af...
<p>Data is presented from 30 subjects (17 in the SSG arm and 13 in TM arm represented in circles and...
<p>a) Viable cells were gated based on negative expression of the fixable viability stain (FVS). Tru...
<p>Healthy human donor PBMCs were mock or VZV infected with or without IL-2 (200 U/ml) for 2 days th...
<p>PBMC were obtained from blood samples and stained for CD45, NK and CD3 with fluorescent antibodie...
<p>PBMC were analyzed post cryo preservation by flow cytometry, to determine the <i>ex vivo</i> freq...
<p>FSc-SSc profile was used to distinguish lymphocytes, monocytes and granulocytes (A). Within the l...
A primary goal of the Denton Immunobiology Lab is improving the killing efficacy of natural killer (...
<p>(A) Gating strategy to distinguish NK cells and subgroups CD56<sup>dim</sup> and CD56<sup>bright<...
<p>A) Percentage values of different cell populations (as indicated, including cytokine-induced kill...
<p>Panels A-D: Lymphocyte subsets among patients included in the randomized KTS-1-2008 study, in the...
<p>(A) Shown are isotype control dot plots and CD14 and CD16 expression in freshly obtained and cryo...
The % of CD3 (CD3+CD19-), the % of CD4 (CD3+CD4+), the % of CD8 (CD3+CD8+) and the % of CD19 (CD3-CD...
<p>T cells were separately analyzed for CD4<sup>+</sup> and CD8<sup>+</sup> subpopulations. NKT cell...
Flow cytometry was used to assess mature B lymphocyte populations for each group. Each graph shows n...
<p>(A and B) Absolute numbers of B cells, CD8 T cells, NK cells and CD4 T cells were re-evaluated af...