Gating strategy used for fluorescence-activated cell-sorter (FACS).

The % of CD3 (CD3+CD19-), the % of CD4 (CD3+CD4+), the % of CD8 (CD3+CD8+) and the % of CD19 (CD3-CD19+) subsets were determined within the total lymphocyte population (gate A = Lympho). For the B cell subsets, the % of CD24++CD38++ transitional B cells and the % of CD24+CD38+ naïve B cells were determined within the total lymphocyte population (gate A = Lympho). The % of IgD+CD27- naïve B cells, the % of IgD+CD27+ unswitched memory B cells and the % of IgD-CD27+ switched memory B cells, and the % of IgD+CD38+/hi activated B cells and the % of IgD-CD38+/low memory B cells were determined within the B cell subset (gate F = CD19+). For the NK cell subsets, the % of CD3-CD56+ NK lymphocytes and the % CD3+CD56+ NK-like lymphocytes were determined within the total lymphocyte population (gate A = Lympho). The % of CD3-CD16++CD56+ naïve cytotoxic NK lymphocytes and the % CD3-CD16+CD56++ active NK lymphocytes were determined within the NK lymphocyte population (gate O4 = CD3+CD56+).