Establishment of 7 kb-Bmp4Luc transgenic mice.

<p>(A) Genomic PCR of transgenic mouse lines (#1, #8 and #17) produced by pro-nuclear microinjection of linearized p7kb-Bmp4Luc. Arrowheads indicate primer sets in the left panel. Template DNA was prepared from tails of each transgenic line and from wild-type mice (Non-Tg) and plasmids (pBmp4-Luc). (B) Fluorescence in situ hybridization (FISH) assays were performed using fibroblast cells isolated from the tail of each transgenic mouse line. p7kb-Bmp4Luc plasmid DNA was used as a probe (yellow signals with arrows). Chromosomal DNA was counterstained with 4′,6-diamidino-2-phenylindole (DAPI) (blue).