Co-localization of NcoA4 and α-tubulin in mammalian cell lines using various NcoA4 antibodies.

<p>COS (<b>A</b> to <b>C</b>) and ES2 (<b>D</b> to <b>F</b>) cells stained for NcoA4 (red) and α-tubulin (green) and examined by confocal and deconvolution microscopy, respectively. These images were generated using a rabbit polyclonal antibody raised against the first 300 amino acids (H300) of NcoA4. Panels <b>G</b> and <b>J</b>: COS cells stained for NcoA4 (red) using H300 antibody. Panels <b>H</b> and <b>K</b>: COS cells stained for NcoA4 (red) using a goat polyclonal antibody (D19). Panels <b>I</b> and <b>L</b>: T47D cells stained for NcoA4 using a mouse monoclonal antibody. Cells were also subjected to DAPI staining for visualization of chromatin (blue). Images shown in panels <b>G</b> to <b>L</b> were generated by confocal microscopy and are of representative cells undergoing metaphase. All images shown are of single optical slices. Yellow immunostaining indicates co-localization of NcoA4 with α-tubulin [Bars are 1.2 µm-10 µm as indicated].