NcoA4 localization and preabsorption with corresponding immunizing peptide and localization of ECFP-tagged NcoA4 at the mitotic spindle.

<p>COS cells were stained for NcoA4 (red) using a goat polyclonal antibody (Q19) and examined by confocal microscopy. Cells were also subjected to DAPI staining for visualization of chromatin (blue). Shown is a representative image of a cell undergoing metaphase (<b>A</b> to <b>C</b>). Immunofluorescence with the antibody preabsorbed with the corresponding immunizing peptide (<b>D</b> to <b>I</b>). COS cells transiently transfected with pECFP-NcoA4 cDNA and stained for CFP-NcoA4 fusion protein using CFP antibody (<b>J</b> to <b>L</b>). All images shown are of single optical slices. [Bars are 2.7 µm to 3.9 µm as indicated].