<p><b>A–B.</b> E-cadherin (Cdh1) localization by immunofluorescence marks blastomere cell-cell adhesion as expected in dsGFP morula (A). E-Cadherin is appropriately localized but present at reduced in dsWdr74 morula (B). <b>C.</b> qRT-PCR assays show reduced RNA polymerase II derived transcripts of <i>Pouf51</i>, <i>Tead4</i>, <i>Actβ</i>, <i>GapdH</i>, <i>Bax</i>, and <i>Cdh1</i> but <i>Trp53</i> shows an increase in transcripts in Wdr74-deficinet embryos. <b>D.</b> The average number of cells in dsGFP and dsWdr74 morula is not significantly different. <b>E–F. </b>Localization of Trp53 by immunofluorescence shows a marked increase of Trp53 protein in dsWdr74 embryos (compare F to E), consistent with the increase in <i>Trp53</i> mRNA. Resul...
<p>(A) Immunostaining of N-cadherin with phalloidin (top panel) and E-cadherin with phalloidin (bott...
<p>(<b>A–C’</b>) Embryos were injected with Par6b-MO together with GFP mRNA into one animal ventral ...
(A) E-cadherin immunofluorescence showing membrane-associated E-cadherin localization (arrows) in H1...
<p><b>A–B.</b> Morphological evaluation of dsWdr74-injected and dsWdr74+dsTrp53 co-injected embryos ...
(A) Immunofluorescence for E-cadherin in embryos at E3.5 (n = 5, a–a”‘), E3.75 (n = 9, b–b”‘), E4.5 ...
<p>(A-C) Blastocelic roof cells of st. 9 animal caps stained for C-cadherin (red in A and B), or E-c...
<p><b>A.</b> Quantitative RT-PCR analysis of endogenous <i>Wdr74</i> mRNA during preimplantation dev...
<p><b>A–H.</b> Immunofluorescence localizes Oct4 and Cdx2 to the inner cell mass and trophectoderm, ...
(A) Relative levels of mRNA for Gata4, Gata6, E-cadherin, N-cadherin, P-cadherin, R-cadherin, VE-cad...
(A) Immunostaining for E-cadherin 24 hours after dsEcad injection. Stars are used to mark the progen...
We report that gene silencing via intracytoplasmic microinjections of morpholino-modified antisense ...
During mammalian embryogenesis the trophectoderm represents the first epithelial structure formed. T...
During mammalian embryogenesis the trophectoderm represents the first epithelial structure formed. T...
<p>(<b>A</b>) Immunostaining of animal caps stained for C-cadherin, showing that the loss of C-cadhe...
<p>(A) Total cell lysates from MDA-MB-435+mock and MDA-MB-435+E-cad were obtained and analyzed by We...
<p>(A) Immunostaining of N-cadherin with phalloidin (top panel) and E-cadherin with phalloidin (bott...
<p>(<b>A–C’</b>) Embryos were injected with Par6b-MO together with GFP mRNA into one animal ventral ...
(A) E-cadherin immunofluorescence showing membrane-associated E-cadherin localization (arrows) in H1...
<p><b>A–B.</b> Morphological evaluation of dsWdr74-injected and dsWdr74+dsTrp53 co-injected embryos ...
(A) Immunofluorescence for E-cadherin in embryos at E3.5 (n = 5, a–a”‘), E3.75 (n = 9, b–b”‘), E4.5 ...
<p>(A-C) Blastocelic roof cells of st. 9 animal caps stained for C-cadherin (red in A and B), or E-c...
<p><b>A.</b> Quantitative RT-PCR analysis of endogenous <i>Wdr74</i> mRNA during preimplantation dev...
<p><b>A–H.</b> Immunofluorescence localizes Oct4 and Cdx2 to the inner cell mass and trophectoderm, ...
(A) Relative levels of mRNA for Gata4, Gata6, E-cadherin, N-cadherin, P-cadherin, R-cadherin, VE-cad...
(A) Immunostaining for E-cadherin 24 hours after dsEcad injection. Stars are used to mark the progen...
We report that gene silencing via intracytoplasmic microinjections of morpholino-modified antisense ...
During mammalian embryogenesis the trophectoderm represents the first epithelial structure formed. T...
During mammalian embryogenesis the trophectoderm represents the first epithelial structure formed. T...
<p>(<b>A</b>) Immunostaining of animal caps stained for C-cadherin, showing that the loss of C-cadhe...
<p>(A) Total cell lysates from MDA-MB-435+mock and MDA-MB-435+E-cad were obtained and analyzed by We...
<p>(A) Immunostaining of N-cadherin with phalloidin (top panel) and E-cadherin with phalloidin (bott...
<p>(<b>A–C’</b>) Embryos were injected with Par6b-MO together with GFP mRNA into one animal ventral ...
(A) E-cadherin immunofluorescence showing membrane-associated E-cadherin localization (arrows) in H1...