Blocking <i>Trp53</i> permits blastocyst formation in Wdr74-deficient embryos.

<p><b>A–B.</b> Morphological evaluation of dsWdr74-injected and dsWdr74+dsTrp53 co-injected embryos at 84 hpf. dsWdr74 embryos do not develop past the morula stage (A). Reduction of <i>Trp53</i> permits differentiation of Wdr74-deficient blastocysts (B). <b>C.</b> Percent of 2-cell embryos reaching the blastocyst stage by 84 hpf in dsGFP, dsWdr74 and dsWdr74+dsTrp53 co-injected embryos. <b>D.</b> qRT-PCR confirms knockdown of <i>Wdr74</i> and <i>Trp53</i> as expected. Results of student T-test shown, error bars represent standard deviation. All data shown normalized to embryo equivalents.