E-cadherin localisation and distribution in E3.5–E5.5 embryos.
- Katarzyna Filimonow (6316724)
- Nestor Saiz (551902)
- Aneta Suwińska (6316727)
- Tomasz Wyszomirski (3555)
- Joanna B. Grabarek (2292745)
- Elisabetta Ferretti (265138)
- Anna Piliszek (6316730)
- Berenika Plusa (6316733)
- Marek Maleszewski (32190)
Publication date
February 2019
DOI Publisher
Public Library of Science (PLoS)Abstract
(A) Immunofluorescence for E-cadherin in embryos at E3.5 (n = 5, a–a”‘), E3.75 (n = 9, b–b”‘), E4.5 (n = 10, c–c”‘), E4.75 (n = 7, d–d”‘) and E5.5 (n = 8, e–e”‘). PrE cells/precursors are GFP-positive (PdgfrαH2B-GFP). The arrow denotes a parietal endoderm cell lacking E-cadherin. Ex–extraembryonic part, Em–embryonic part. (B) Scheme for measuring fluorescent signal to determine the relative levels of E-cadherin in E3.5, E3.75 and E4.5 embryos. Red lines indicate measurement of fluorescent intensity between two Epi and two PrE cells. (C) Estimated levels of E-cadherin in PrE cells relative to Epi cells in E3.5, E3.75 and E4.5 embryos; 95% confidence intervals are plotted for the ratios.</p
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