<p>A. Bulk expression of H3K56ac analyzed by immunoblotting. Actin is the loading control. B, C. ChIP analysis of H3 occupancy at DDR gene promoters in response to HU (one hour). Immunoprecipitated DNA was normalised to input DNA and signal obtained in untreated wild type cells was set to 1. D, E. ChIP analysis of H3K56ac occupancy at DDR gene promoters in the indicated strains grown as in B and C. Normalization of the data is described in the <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0021633#s4" target="_blank">methods</a> section. In B-E, error bars show standard deviation between three biological replicates.</p
<p>(<b>A</b>) HEK293IL-1R cells were transiently transfected with a vector directing the synthesis o...
<p>(<i>A</i>) <i>In situ</i> hybridization experiments illustrating the expression of the <i>BAN</i>...
<p>(<b>A</b>) Venn diagram showing genes where transcript levels changed significantly (p<0.01) in r...
<p>A. ChIP analysis of Snf2 recruitment to the promoter of <i>RNR3</i> in response to HU. In this an...
<p>ChIP assays for the active H3K4me3 and repressive H3K27me3 histone modifications were carried out...
<p>(<b>A</b>) MCF-7 cells stably expressing either a non-specific shRNA (shRNA<sup>Con</sup>) or shR...
<p>(A) RT-qPCR analysis of <i>HCF173</i> mRNA levels in wild-type and in <i>hub1-3</i> mutant seedli...
<p><b>(A</b>) Correlation of H3K4me3 signal at promoters and gene expression in control and HD. X-ax...
<p>A. Gel image representative of three (n = 3) independent experiments for sqRT-PCR of <i>HYPK</i> ...
<p>A) Transcript levels of <i>WRKY70</i> and <i>LTP7</i> genes in wild type (WS), two different <i>a...
<p><b>A</b>) Immunoblotting for H4K20me2, H4K20me1, and H4 (loading control) in individually derived...
<p>At two (upper panels) and five days (lower panels) post siRNA injection, mouse livers were subjec...
<p>ChIP analysis of ΔE3A-LCL<sup>doxE3A</sup> cells showing the abundance of (A) Pol II, (B) H3ac, (...
<p><b>A.</b> ChIP analysis of YFP from cells expressing wild type H3.3-YFP or H3.3 K56Q-YFP or H3.3 ...
<p>(A) SWI/SNF binding to the <i>HTA1-HTB1</i> promoter in synchronized cultures of wild-type (blue ...
<p>(<b>A</b>) HEK293IL-1R cells were transiently transfected with a vector directing the synthesis o...
<p>(<i>A</i>) <i>In situ</i> hybridization experiments illustrating the expression of the <i>BAN</i>...
<p>(<b>A</b>) Venn diagram showing genes where transcript levels changed significantly (p<0.01) in r...
<p>A. ChIP analysis of Snf2 recruitment to the promoter of <i>RNR3</i> in response to HU. In this an...
<p>ChIP assays for the active H3K4me3 and repressive H3K27me3 histone modifications were carried out...
<p>(<b>A</b>) MCF-7 cells stably expressing either a non-specific shRNA (shRNA<sup>Con</sup>) or shR...
<p>(A) RT-qPCR analysis of <i>HCF173</i> mRNA levels in wild-type and in <i>hub1-3</i> mutant seedli...
<p><b>(A</b>) Correlation of H3K4me3 signal at promoters and gene expression in control and HD. X-ax...
<p>A. Gel image representative of three (n = 3) independent experiments for sqRT-PCR of <i>HYPK</i> ...
<p>A) Transcript levels of <i>WRKY70</i> and <i>LTP7</i> genes in wild type (WS), two different <i>a...
<p><b>A</b>) Immunoblotting for H4K20me2, H4K20me1, and H4 (loading control) in individually derived...
<p>At two (upper panels) and five days (lower panels) post siRNA injection, mouse livers were subjec...
<p>ChIP analysis of ΔE3A-LCL<sup>doxE3A</sup> cells showing the abundance of (A) Pol II, (B) H3ac, (...
<p><b>A.</b> ChIP analysis of YFP from cells expressing wild type H3.3-YFP or H3.3 K56Q-YFP or H3.3 ...
<p>(A) SWI/SNF binding to the <i>HTA1-HTB1</i> promoter in synchronized cultures of wild-type (blue ...
<p>(<b>A</b>) HEK293IL-1R cells were transiently transfected with a vector directing the synthesis o...
<p>(<i>A</i>) <i>In situ</i> hybridization experiments illustrating the expression of the <i>BAN</i>...
<p>(<b>A</b>) Venn diagram showing genes where transcript levels changed significantly (p<0.01) in r...