Changes in transcript level induced by HDACi.

<p>(<b>A</b>) Venn diagram showing genes where transcript levels changed significantly (p<0.01) in response to HDACi, detected by HGMP 5K array (5000 gene probes). HL60 cells were treated with VPA (5 mM), SAHA (2.5 µM) or TSA (165 nM) for eight hours. (<b>B</b>) RT-qPCR was used to quantify the transcriptional change at genes that responded to all three HDACi. HL60 cells were treated with VPA (5 mM, 8 hours). Transcript abundance was normalised to β-actin, and presented on a base 2 logarithmic scale. Values are presented with a standard error of mean (<i>n = 3</i>). (<b>C</b>) Cells were treated with VPA (5 mM), and transcript abundance analysed at defined time points using <i>Operon</i> microarrays (25823 genes). Quantitative presentation of the number of genes that were more than 2-fold up-regulated (<i>Shaded bars</i>) or down-regulated (<i>Filled bars</i>) at time points during the time course. (<b>D</b>) RT-qPCR was used to quantify the transcriptional change at genes that responded to VPA (<i>5 mM</i>) at 1, 2 and 8 hours treatment. Genes show divergent responses, including early and continued up-regulation (<i>ANXA2, DLK1, IL1B, KIF3C</i>), little initial change and subsequent down-regulation (<i>MCM7, PES1, LMO2</i>) or early and persistent down-regulation (<i>MYC</i>). Transcript abundance was normalised to β-actin, and presented on a base 2 logarithmic scale.