HRas activation increases phosphorylation of MLC.

<p>Influenza-induced MLC phosphorylation is inhibited by inhibiting HRas, RhoA, and PKC. (A, B) HUVECs were transduced with the indicated adenoviruses and infected 48 h later with influenza virus (MOI 10). The cells were collected 8 h later for blotting. Limax flavus lectin (LFL)–induced MLC and ERK phosphorylation depends on HRas, Raf-1, PKC-α, and RhoA. (C, D) HUVECs were transduced with the indicated adenoviruses; 48 h later, the cells were treated for 15 min with LFL [100 mg/ml]. Activation of HRas, RhoA, and PKC-α leads to MLC and ERK phosphorylation. (E, F) Western blots performed 48 hours after HUVECs were transduced with empty adenovirus vector (Null) or the indicated adenoviruses. *P<0.01; N = 4 for each experiment.