Higher polymerase activity of a human influenza virus enhances activation of the hemagglutinin-induced Raf/MEK/ERK signal cascade-3

<p><b>Copyright information:</b></p><p>Taken from "Higher polymerase activity of a human influenza virus enhances activation of the hemagglutinin-induced Raf/MEK/ERK signal cascade"</p><p>http://www.virologyj.com/content/4/1/134</p><p>Virology Journal 2007;4():134-134.</p><p>Published online 5 Dec 2007</p><p>PMCID:PMC2222635.</p><p></p>ion was analyzed with a mAb specific for phosphorylated ERK (P-ERK). Subsequently, loading was controlled with a mAb against ERK2. Respective bands of three independent experiments were quantified, and relative ERK activation was calculated and normalized to the loading control (mock-infected, white bar). Virus types as well as the time of analysis post-infection (p.i.) are indicated. (B) MDCK cells were infected at m.o.i. = 1 either with H1N1 ± TPA or with H3N2 ± U0126, and the supernatant was harvested 9 h later. The mean virus titers are given in percent as well as plaque forming units/ml. The error bars were derived from three independent experiments