Screening host factors that regulate influenza virus polymerase activity.

(A) Host proteins were selected to analyze their roles in polymerase activity (PB2627E or PB2627K) of influenza virus H7N9 (A/Anhui/1/2013). The log2 logarithmic values of the ratio of the luciferase activity transfected with the corresponding host factors and the empty pCAGGS plasmids were used as the PB2627E or PB2627K polymerase activity. (B) Host proteins from avian were selected to analyze their roles in polymerase activity (PB2627E) in HEK293T cells. RBM10-ck v1 and RBM10-ck v2, and RBMX-ck v2 and RBMX-ck v3 are two different transcripts of avian-derived RBM10 and RBMX, respectively. The detailed base sequences are in the attached S3 Table. Statistical differences between groups are labeled according to a one-way ANOVA followed by a Dunnett’s test. Each treatment was repeated three times in parallel. The results are presented as means ± standard deviations. *, P < 0.05; ***, P < 0.001; ****, P < 0.0001; ns, no significance. ck, chicken; hu, human.